GPT and acute kidney injury: The toxic effects of 1 and 0.75% ethylene glycol can result in the violation of liver functions, the levels of ALT, AST, ALP increase in the blood serum, acute renal failure, which ultimately leads to high mortality of animals (Yamaguchi et al., 2005; Breljak et al., 2015).When using EG 0.5%, by the 3rd week of the study, we did not find stones in the lumen of the renal tubules, in the pyelocaliceal system, in the ureters and in the lumen of the bladder.