Taken together, our study suggested that a two-compartment model with first-order elimination was the best fit for PPK of PEM in Chinese primary advanced NSCLC patients, along with CrCl as an essential covariate on CL, ERCC1 (rs3212986) and CYP3A5 (rs776746) gene polymorphisms as essential covariates on Q. The prediction accuracy of the final model was reliable and the stability of the final model was good, which were indicated via GOF plots, non-parametric bootstrap and VPC. Here, CYP3A5 is linked to non-small cell lung carcinoma.