Then, the immune landscape across lung cancer subtypes was depicted, including the immune scores calculated by ESTIMATE (Yoshihara et al., 2013), abundances of 28 subpopulations of infiltrating immune cells quantified by single-sample gene set enrichment analysis (Subramanian et al., 2005) (Supplementary Material), and mRNA expression levels of three immune checkpoint genes (PD-1, PD-L1, and CTLA4). The gene discussed is CTLA4; the disease is lung carcinoma.