Besides, in bufothionine-treated SMMC-7721 hepatocellular carcinoma cells, it could be observed that the concentration of IL-6 was decreased, the expression of p-Stat3-tyr705, p-Stat3ser727, and JAK2 was downregulated, as well as there was an increase in Atg5, Atg7, and LC3-II. The gene discussed is STAT3; the disease is hepatocellular carcinoma.