In addition to inducing autophagic death of cancer cells by regulating the expression of autophagy-related markers p62 and LC3-II, I3C, when used in association with HCQ, can induce apoptosis of EAC cells by activating cleaved caspase-3 and Bax while inhibiting the Bcl-2 protein level, thus prolonging the survival time of mice. This evidence concerns the gene SQSTM1 and cancer.