Through BN-PAGE of isolated mitochondria from wildtype Neuro2a cells (Con), and Neuro2a cells that had been engineered to stably express either wildtype APP (APPwt) or the APP Swedish mutation associated with AD (APPswe) [61], we observed APP-dependent decreased CHCHD6 within the MICOS (Fig. 1a). This evidence concerns the gene CHCHD6 and Alzheimer disease.