BN-PAGE of mitochondrial protein lysates from the hippocampus of APP KI or WT mice at 3, 6 and 9 months of age, followed by western blotting for MICOS core proteins, showed AD-specific and age-related decrease in hippocampal CHCHD6 on the MICOS, which was absent in WT mice and most severe in rapidly disease developing APPNL−G−F KI mice (Fig. 1d). The gene discussed is PROS1; the disease is Alzheimer disease.