Consistent with our prior observations, and those of others, that eprenetapopt triggers GSH depletion in cancer cells (7, 8), the CRISPRko screen identified genes involved in GSH synthesis, SLC7A11 and GCLM, while GCLC was the top enriched gene in the CRISPRa screen (Fig. 1D). This evidence concerns the gene GCLM and cancer.