Finally, an unsupervised analysis of scRNA-Seq profiles of immune cells isolated from 48 tumor biopsies taken either at baseline or during treatment with ICB (73) confirmed enrichment of PI24 scores in immune cells (NK cells, effector memory, and central memory CD4+ and CD8+ T cells) from pretherapy lesions of nonresponders, i.e., patients with progressive or stable disease, compared with responders (complete or partial response; Supplemental Figure 29, A and B). The gene discussed is CD4; the disease is neoplasm.