Quantitative real-time PCR on SMN2 transcripts in mouse retinas showed a significant, 5.5-fold increase in the full-length SMN2 transcripts (P < 0.0001) and a 37% decrease in the SMN2 transcripts lacking exon 7 (Δ7 SMN2) (P = 0.0005) in PMO25-treated SMA mice compared with untreated SMA controls (Figure 3A), suggesting the high efficacy of PMO25 in augmenting SMN2 exon 7 splicing. The gene discussed is SMN2; the disease is proximal spinal muscular atrophy.