We sorted granulocytes (CD45+CD11b+CD3−CD15+CD14−), T cells (CD45+CD3+CD11b), and monocytes (CD45+CD3−CD14+CD15−CD11b+CD11c+) from APS and WBCs to evaluate the mechanistic pathway activity (FACS gating strategy shown in Fig. S3). The gene discussed is ITGAX; the disease is autoimmune polyendocrinopathy.