We used flow cytometry to investigate the immune cell types present in APS; we defined populations of neutrophils (CD45+CD3−CD15+CD16+), monocytes (CD45+CD3−CD15−CD14+CD16dim), natural killer cells (NK) cells (CD45+CD3−CD15−CD14−CD16+HLA−), NKT cells (CD45+CD3+CD14−CD16+), and T cells (CD45+CD3+CD14−CD16−) based on cell surface markers, gating strategy shown in Fig. S1a. Here, PTPRC is linked to autoimmune polyendocrinopathy.