We also knocked down CKS2 with two shRNAs in human retinoblastoma WERI-Rb-1 cells (Supplemental Fig. S10A) and found that downregulation of CKS2 in this cell type led to similar phenotypes as well including cell proliferation, colony formation and EdU staining (Supplemental Fig. S10B–D). Here, RB1 is linked to retinoblastoma.