According to the results of Mann–Whitney test, we did not observe any statistically significant difference in clinical characterize (menstrual interval, hirsutism and acne scores, waist and hip circumstances and weight) and endocrine laboratory tests (LH/FSH ratio, testosterone, insulin and FBS) before and after of intervention of participants between groups (P > 0.05). Here, PLOD1 is linked to Hirsutism.