Besides, the lipid content in the liver tissues of the NAFLD mice after the treatment of EVs-inhibitor NC + LV-sh-NC was reduced, which was rescued after miR-223-3p inhibition in the NAFLD mice, and further E2F1 knockdown reduced the lipid content (Figure 7g-h, Fig. S5C-D). The gene discussed is E2F1; the disease is metabolic dysfunction-associated steatotic liver disease.