The ELISA suggested that ALT and AST levels were up-regulated in serum of NAFLD mice, and the up-regulation was reversed in response to additional treatment by EVs-mimic NC or EVs-miR-223-3p mimic; and EVs-miR-223-3p mimic led to a more obvious reversion relative to EVs-mimic NC (Figure 3b-c). The gene discussed is GPT; the disease is metabolic dysfunction-associated steatotic liver disease.