These findings are supported by previous reports that demonstrated NACs ability to improve Pdx-1 and insulin content in beta-cells in a genetic obesity model of T2DM (26), and to prevent glucose toxicity-induced impaired Pdx-1 binding to the insulin gene promoter in beta-cell line in vitro and reduce plasma 8-OHdG oxidative stress level in Zucker diabetic fatty rats (41). This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.