To investigate the role of RP11-59J16.2 and MCM2 in the development of AD in vitro, SH-SY5Y cells treated with 10 μM Aβ 1–42 were transfected with NC, si-RP11-59J16.2, MCM2, si-RP11-59J16.2 + si-NC, si-RP11-59J16.2 + si-MCM2. Here, MCM2 is linked to Alzheimer disease.