The proportions of 8 immune cells in 85 Ewing sarcoma patients and 3 normal samples were presented by the bar plot, encompassing B cells, cancer-associated fibroblasts, CD4+ T cells, CD8+ T cells, endothelial cells, macrophages, NK cells, and uncharacterized cells (Figure 3A). The gene discussed is CD4; the disease is Ewing sarcoma.