To explore the biological function of ST3GAL6 in the LUAD development, we first selected LUAD cell line A549 with wild-type EGFR expression and an EGFR-mutant (exon 19 deletion) LUAD cell line HCC827 (Tracy et al., 2004) to deplete the endogenous ST3GAL6 expression, according to the expression level of ST3GAL6 in a non-small cell lung cancer cell line from the CCLE database for STG3AL6 knockdown by two short hairpin RNAs (shRNAs) (Supplementary Figure S2A). The gene discussed is EGFR; the disease is non-small cell lung carcinoma.