In summary, we found that ST3GAL6-AS1 positively regulates ST3GAL6 in LUAD cells, while the depletion of ST3GAL6-AS1 activates the EGFR signaling pathway, upregulates the expressions of MMP2 and 9, and promotes the invasion of LUAD tumor cells. The gene discussed is ST3GAL6; the disease is neoplasm.