Clinically, the hepatotoxicity caused by TwHF and its preparations containing TP is mainly manifested as acute hepatitis, with symptoms such as nausea, fatigue, anorexia, increased serum alanine transaminase (ALT)and aspartate aminotransferase (AST) levels, and obvious cholestasis (Yang et al., 2018). This evidence concerns the gene GPT and cholestasis.