The current study did not specifically address the role of a reduction in RBF on RAAS activation in CHF; however, previous studies have shown that CHF is associated with decreased RBF (Clayton et al., 2011), increased ANG II type 1 receptor (AT1R) expression and decreased ANG II Type 2 receptor expression in the kidney (Clayton et al., 2011; Peng et al., 2020) and that reducing sympathetic stimulation of the kidney by renal denervation prevents these changes (Clayton et al., 2011). Here, AGTR1 is linked to congestive heart failure.