WT1 and mesothelioma: IHC staining of mesothelioma related markers including BAP1, EMA, Thrombomodulin (CD141), WT1, Podoplanin (D2-40), Calretinin and Cytokeratin6 (CK-6) revealed increased protein expression in 2175, 1187 or 1157 3D spheroids when compared to their 2D counterparts (Figure 3).