In the groups of GPC-3 positive and negative HCC, the tumor size were 3.73 ± 2.7 cm and 4.4 ± 2.8 cm, respectively (P=0.091); the number of patients presenting nonperipheral “washout” were 182 (87.5%) and 65 (92.9%), respectively (P=0.095); the number of patients presenting “infiltrative appearance” were 28 (13.5%) and three (4.3%), respectively (P=0.046); the number of patients presenting “marked diffusion restriction” were 87 (41.8%) and 17 (24.3%), respectively (P=0.01); the number of patients presenting “iron sparing in solid mass” were 34 (16.3%) and 18 (25.7%), respectively (P=0.11). The gene discussed is GPC3; the disease is hepatocellular carcinoma.