Univariate Cox regression demonstrated the pretreatment GSVA score of ccRCC.Sig, PBRM1 mutation and intratumor heterogeneity (ITH) were closely related to poorer OS (Figure 5H), and multivariate Cox regression found that ccRCC.Sig, wild-type PBRM1 (20, 46, 59) and ITH were independent risk factors (Figure S12K). The gene discussed is PBRM1; the disease is nonpapillary renal cell carcinoma.