Remarkably, we found that more CD8+ T-cells were positive for Notch1 in ZM-treated organoids than in controls (Figure 2A) and ZM-treated organoids displayed increased IFN-gamma production, higher infiltration and clusters of CD8+ T-cells surrounding cancer cells, instead of the dispersed organization of T-cells observed in control organoids (Figure 2A and Supplementary Figure 2A). This evidence concerns the gene IFNG and cancer.