After CKAP2L knockdown in ESCC cells, the results of western blot analysis validated that CKAP2L induced the expression of cyclin B1 and CDK1, whereas CKAP2L-OE up-regulated the expression of cyclin B1 and CDK1 in comparison to the Vector group (Figure 5(e)). The gene discussed is CCNB1; the disease is esophageal squamous cell carcinoma.