In addition, this protocol also induces reduction in the tyrosine hydroxylase (TH) labeling, increase in lipid peroxidation and increased alpha-synuclein levels in the nigro-striatal dopaminergic pathway (Fernandes et al., 2012; Santos et al., 2013; Leão et al., 2017), which are important factors related to PD pathophysiology. This evidence concerns the gene SNCA and Parkinson disease.