We chose the well-characterized murine cancer testis (CT)26 and MC38 cell lines to model the effects of ionizing radiation on changes in the proteome and MHC-presented immunopeptidome in colorectal cancer cells to investigate the pathways driving MHC class I upregulation, which could be translated from pre-clinical studies to human clinical trials (14). The gene discussed is HLA-C; the disease is colorectal cancer.