The typical EMT phenotypes, down-regulation of E-cadherin and ZO-1, and up-regulation of N-cadherin and MMP2, were found as well in IGSF9-knowdown breast cancer cells with western blot analysis (Fig. 4A, B) and immunofluorescence assay (Fig. 4C–E). This evidence concerns the gene TJP1 and breast carcinoma.