The median percentage of PD-L1 positive circulating T lymphocytes was 1.4% in patients with lymph node metastasis, significantly higher than that in patients without lymph node metastasis (0.6%, Table 1); The median percentage of PD-L1 positive circulating T lymphocytes in HER-2 positive patients was 0.5%, significantly lower than that in HER-2 negative patients (0.9%, Table 1). The gene discussed is CD274; the disease is metastatic malignant neoplasm in the lymph nodes.