The treatment with SAL triggered phosphorylation of Akt (Figure 4(A)) and GSK-3β (Figure 4(B)), and the expression of pSer473-Akt and pSer9-GSK-3β was suppressed in the kidney of rats with DN but had no effect on protein levels of Akt and GSK-3β (p < 0.05). This evidence concerns the gene AKT1 and liver dysplastic nodule.