To further elucidate whether the suppression of glioma progression is mediated through the miR-3591-3p–MAPK1 axis, we overexpressed MAPK1 in glioma cells and performed gain-of-function experiments to verify whether MAPK1 was able to rescue the phenotypic effects of miR-3591-3p. The gene discussed is MAPK1; the disease is central nervous system cancer.