Based on the selected starting point of the developmental stage, the naïve marker CCR7 was decreased, the cytotoxic effector (GZMA, GZMK) and MS susceptibility genes (HLA-A, HLA-DQB1, HLA-DRA, HLA-DRB1, and RGS1) were increased along with the increase of pseudotime (Fig. 4h), indicating their crucial role in involving in disease pathogenesis. This evidence concerns the gene HLA-A and myeloid sarcoma.