After comparing the differential gene expression, the volcano plot (Fig. 4a; Supplementary Data 4) showed that T cells in TDL upregulated genes associated with immunity (GZMK, GZMA, and CXCL13) and MS susceptibility gene RGS1 and downregulated genes associated with myelination (PLP1), glial cell differentiation (GFAP), and tumor cell proliferation (SOX2-OT). Here, SOX2 is linked to neoplasm.