There were significant differences between primary and metastatic tumor cells throughout the sections, with 12 of 25 (48%) and 3 of 6, STAT3 was not expressed in either cell; whereas 12% and 16% of primary and metastatic tumor cells, respectively, showed positive expression of Nrf2, were positive for Nestin expression, and none of the cells tested positive for SOX2. This evidence concerns the gene NES and metastatic neoplasm.