As shown in Table 2, a significant relationship was found among TFR1 expression and age (P=0.001), Lauren type (P=0.008), T stage (P=0.003), HER2 (P=0.003), PD-L1 (P < 0.001), and the level of CA72-4 (P=0.028), while TFR1 expression was not significantly associated with gender, tumor location, Borrmann type, differentiated degree, N stage, and TNM stage. This evidence concerns the gene CD274 and neoplasm.