In addition, a number of experimental factors could be involved, such as the mouse model, gender, age, and the type of atherogenic diet, along with the high-fat diet feeding time (84), as in female Ldlr–/– mice, Nlrp3 deficiency in bone marrow cells is associated with decreased atherosclerosis but not in male mice (85). This evidence concerns the gene NLRP3 and atherosclerosis.