Because MAGI1 induces EC activation (20), we investigated whether IAV activated ECs through upregulation of MAGI1 by determining relative changes in mRNA expression level of intercellular adhesion molecule 1 (ICAM-1), a marker of EC activation, using qRT-PCR in MAGI1-depleted HUVECs with or without virus infection. This evidence concerns the gene ICAM1 and viral infectious disease.