Yang et al. (2021) also indicated that Mesenchymal stem cells-conditioned medium (MSC-CM) enhanced the biological functions of mitochondria, inhibited inflammation, and prevented cell apoptosis both in vivo and in vitro, which significantly improved NAFLD. These positive effects were closely related to the upregulation of SIRT1. SIRT1 has been reported to have anti-steatosis and anti-inflammatory movements in the pathogenesis of NAFLD (Meng et al., 2020). This evidence concerns the gene SIRT1 and metabolic dysfunction-associated steatotic liver disease.