CF epithelial cell cultures using CFTR ΔF508 CFBE41o- mutant polarized epithelial cells have been used to investigate the human CF airway epithelial microenvironment with the most common CF pathogen, P. aeruginosa, to better understand the persistent colonization of the CF airway with this pathogen (Moreau-Marquis et al., 2008). Here, CFTR is linked to cystic fibrosis.