Finally, according to the rescue assay, inhibiting CDKN2B-AS1 expression can enhance the autophagy ability of LC cells and also greatly slow down their growth ability, while suppression of miR-199a-5p expression can completely reverse the impacts of CDKN2B-AS1 on the cell migration, invasion, proliferation, apoptotic activity, and autophagy ability. Here, CDKN2B is linked to laryngotracheoesophageal cleft.