The indispensable role of NOX was shown by using the NOX2 inhibitor DPI to significantly reduce PMA-induced NETosis, and that neutrophils from chronic granulomatous disease (CGD) patients—that have mutations in one of the genes encoding the components of the Nox2 NADPH oxidase complex—lose their ability for NETosis (19). This evidence concerns the gene CYBB and chronic granulomatous disease.