Splenocytes, natural killer (NK) cells, human liver cancer cells (HepG2) [77], human gastric cancer cells (AGS), human colorectal cancer cells (HCT) and human non-small-cell lung cancer calls (A549) [77,78] were selected as the experimental models to verify the anti-tumor activity of Fructus Malvae. Splenocyte proliferation ability, natural killer (NK) cell activity, AGS cell apoptosis percentage, and the expression of the apoptosis proteins PARP, Cleaved APRP, Caspase-3, Cleaved Caspase-3, Bcl-2, Bax, β-actin, etc. were detected as indicators in the study [33,34]. Here, CASP3 is linked to liver cancer.