Furthermore, vactosertib disturbed TGF-β induced by EMT and Akt signaling, leading to significant reductions in the motility and growth independent of anchorage of two HCC cell lines, SK-HEP1 and HepG2 cells, as indicated in wound-healing and soft agar colony formation assays [208]. This evidence concerns the gene AKT1 and hepatocellular carcinoma.