Interestingly, incubation of Huh-7 cells with oleate in combination with palmitate prevented ER stress-related gene expression (Figure 4C–G) but was unable to reverse palmitate-induced insulin resistance, as was depicted by reduced ERK phosphorylation (Figure 4A), and the lessened glucose uptake (Figure 4B) after insulin treatment in these cells. This evidence concerns the gene INS and Insulin resistance.