HMGB1 and hydrops fetalis: In offspring, maternal HF diet increased expression of these inflammatory genes in both male (TNFα, IL-1β, IL-6, CD3, TLR2, TLR4 and Rage) and female (TNFα, IL-6, CD3, CD68, Hmgb1, TLR2, TLR4 and Rage) offspring on PND21, while maternal MT treatment normalized the expression of these genes (except for CD68 in females) (Figure 4B,C).