Histological staining and quantitative analysis in Figure 7 and Figure 8 show that after three days of implantation, decreased inflammatory cell counts, TRAP+ cell number and iNOS+ cells and increased CD163+ cells were observed compared with the Ti group, although no difference was detected between the B-TNT/PDA and B-TNT/PDA/IL-4+Laser groups. The gene discussed is IL4; the disease is Patent ductus arteriosus.