In our study we show that the treatment with Ac2–26, one hour before the CHIKV infection and every 24 h for the next 3 three days, reduced the levels of CXCL1 and decreased the neutrophil count in the paw of CHIKV-infected mice, without interfering with the viral titers at the site of infection (paw/ankle joint) and independently of viral infectivity. This evidence concerns the gene CXCL1 and infection.