Entry into the S-phase of the cell cycle is significantly inhibited by VNPP433-3β in addition to impeding several oncogenic pathways such as WNT signaling, cyclin and cell cycle regulation, c-AMP mediated signaling, STAT3 pathway, glioblastoma multiforme and basal cell carcinoma signaling, CREB signaling, etc. Therefore RNA-seq data further validates the PCa-inhibiting effects of VNPP433-3β observed in the biochemical studies besides revealing the significant pathways that are modulated. Here, CREB1 is linked to glioblastoma.