By performing sc-RNAseq during the differentiation process from myoblast to myotubes on both FSHD and control-derived cells, a proportion of DUX4 targets (MBD3L2, TRIM43, LEUTX and ZSCAN4) were detected as overexpressed in FSHD subjects [98]. The gene discussed is MBD3L2; the disease is facioscapulohumeral muscular dystrophy.