VEGFA and peripheral arterial disease: VEGF165b inhibition enhances perfusion in Type-2 diabetic-PAD mice. Immunoblot analysis to determine the relative levels of VEGF165b and total VEGF-A showed no significant differences between a non-ischemic high-fat diet (HFD, 60 kCal for 4 months) vs. normal chow-fed mice skeletal muscle samples (Supplementary Figure S4A).