Using a microfluidic device, in which melanoma cells and splenocytes were separated by a microchannel, IRF8 KO splenocytes exhibited a marked decrease in the ability to cross the channel to infiltrate the melanoma cells while melanoma cells exhibited marked propensity to invade the channels suggesting existence of an IRF8-regulated communicating soluble factor [174]. This evidence concerns the gene IRF8 and melanoma.