A study done by Ricupito et al., assessing dendritic cell-based vaccines in both prophylactic as well as therapeutic settings with different prime-boost regimens reported that booster vaccinations were important for the maintenance of Ag-specific CD8+ TCM cells in the prophylactic setting, however frequent boosting intervals with up to three booster vaccines hindered cell survival/functionality of TCM cells in the therapeutic setting, suggesting that different boosting schedules affect the outcome of memory T cell differentiation when a tumor was present versus absent [29]. This evidence concerns the gene CD8A and neoplasm.