Representative images of CD3 and CD8 immunostaining of the IChigh subgroup (an MSS CRC) are shown in Figure 4A. Regarding IC expression, positive cells could be quantified for PD-1, TIGIT and CD94; however, they could not be quantified for Lag3 as it was barely expressed by TILs in almost all cases, or Tim-3, which was difficult to quantify—specifically for lymphocytes—as it was also expressed by other immune cells. The gene discussed is PDCD1; the disease is colorectal carcinoma.