In addition, our findings highlight a significantly higher frequency of TILs expressing not only PD-1 but also TIGIT and Tim-3; this is associated with an increased expression level in terms of MFI for TIGIT and PD-1 in MSI versus MSS CRCs, and accordingly, a slightly higher expression level in the CMS1 CRC encompassing mostly MSI CRC. This evidence concerns the gene PDCD1 and colorectal carcinoma.