We then performed a quantitative in situ assessment of the CD3+ and CD8+ T cells, as well as IC-expressing cells, using immunohistochemistry on paraffin sections from the tumors of cohorts 2 and 3 (n = 31, 26 MSS, 5 MSI), both inside the tumor glands and in the peritumoral stroma. Here, CD8A is linked to neoplasm.