However, when examining the CMS subgroups, significant differences were observed in the frequency of CD4+ TILs in “IChigh” clusters 10 (Kruskal–Wallis test, p = 0.0342) and 11 (Kruskal–Wallis test, p = 0.0464) in immunogenic CMS1 tumors encompassing the majority of MSI CRC (Figure S3D). Here, CD4 is linked to colorectal carcinoma.